크로마토그래피 원리의 큰 틀도 마찬가지로 두 상에 대한 분배 차이를 이용하여 분석물을 분리, 정제할 수 있습니다. 다만 크로마토그래피에서 두 개의 상은 하나는 고정하고 다른 하나는 일정 방향으로 이동시켜 사용합니다.
Integrator is the computer-dependent facts processor accustomed to document the electronic sign. Uncomplicated to specially designed computer software is formulated for HPLC.
物質の濃度により光の通過する角度が変わることを利用した検出器。原理上グラジェント分析はできない（グラジェントによって移動相自体の屈折率が変化するため）。また、感度が低いのが欠点だが、大部分の物質に対して使用できる。
Within this part we evaluate the standard plumbing necessary to shift the mobile stage throughout the column and to inject the sample to the cellular period.
物質にエネルギーを与える（励起）ことにより発光する（蛍光）性質を利用した検出器。一般に選択性が高く高感度で、物質に特異的な検出が可能。蛍光する性質を持たない物質については、その物質を標識することにより検出が可能になる。
A detector identifies and actions each component. Retention time signifies time taken for every compound to exit the column. HPLC's effectiveness is determined by things like column sort and cell period composition. Common maintenance guarantees accurate outcomes. Comprehension HPLC's move-by-move course of action is significant for specific chemical Assessment in laboratories.
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The info acquisition system information and processes the signals in the detector, enabling with the creation of chromatograms and the quantification of compounds.
An HPLC commonly contains two columns: an analytical column, which is answerable for the separation, in addition to a guard column that's placed prior to get more info the analytical column to guard it from contamination.
Dimensions-exclusion chromatography, generally known as gel filtration or gel permeation chromatography, separates substances determined by their dimension and molecular fat. Smaller molecules can penetrate the porous construction of your stationary phase and elute more rapidly, even though greater molecules are held for a longer period.
Two challenges usually shorten the life time of an analytical column. First, solutes that bind irreversibly to your stationary section degrade the column’s performance by decreasing the level of stationary period readily available for effecting a separation. Next, particulate substance injected Together with the sample could clog the analytical column.
Column selection: The stationary section from the column interacts with analytes. Using the Incorrect column chemistry may result in lousy resolution. Think about using a distinct column using a stationary section that provides improved selectivity on your analytes.
While using the check here Investigation approach understood, let us address prevalent troubles that may crop up and how to troubleshoot them.